High performance Essay

ConclusionThere are 3 different component dyes in the mix including blue dye, lily-livered dye and red dye. The blue dye has the highest Rf in chromatography ( Rf=1 in water and ethanol and isoprophy alcohol). The yellow dye has the lowest Rf in chromatography in ethanol and isoprophy alcohol and middle Rf in water (Rf=0.17 and isoprophy alcohol and lowest Rf in water (Rf=0.816 in ethanol, Rf= 0.678 in water and Rf=0.680 in isoprophy alcohol).Real World ConnectionHPLC-MS stands for High performance liquid chromatography mass spectrometry. HPLC-MS shares the same(p) principle with paper chromatography. They both separate mixture using the difference in different affinities to mobile/stationary phase of different components. However, HPLC-MC is far different from paper chromatography. Firstly, HPLC-MC is composed of a liquid chromatography and a mass spectrometer. This instrumentation enables HPLC-MC to analyze a much wider range of components. While paper chromatography only qualita tively separate different component in a liquid mixture, HPLC-MS can quantitatively examine each component, including Compounds that are thermally labile, exhibit high signal or have a high molecular mass.Secondly, paper chromatography use solvent as mobile phase and paper as stationary phase, further in HPLC-MS the mobile phase is the mixture of liquid and the stationary phase is the self-coloured through which liquid flow. Thirdly, paper chromatography can be carried out at normal temperature and pressure, but HPLC-MS sometimes needs to be done under high pressure and certain temperature to successfully drive the liquid through the solid and best demonstrate the difference in affinities of different liquid compound.